NRCM from Rattus norvegicus Phenylephrine (50µM)_Isoproterenol (10µM)... - SRA

SRX1013277: NRCM from Rattus norvegicus Phenylephrine (50µM)_Isoproterenol (10µM) treatment 24h
3 ILLUMINA (Illumina HiSeq 2500) runs: 42.1M spots, 4.2G bases, 1.7Gb downloads

Design: Total RNA was isolated from NRCMs with the RNeasy Mini Kit (Qiagen). Starting material for library preparation was 500 ng total RNA. mRNA was isolated using magnetic beads (NEBNext® Poly(A) mRNA Magnetic Isolation Module, NEB). Library preparation was performed with NEBNext® UltraTM RNA Library Prep Kit for Illumina®. 13 PCR cycles were used for library enrichment. Agencourt® AMPure® XP Beads (Beckman Coulter) were used for purification and size selection.

Submitted by: University of Freiburg

Study: Rattus norvegicus Genome sequencing

PRJNA281569 • SRP057783 • All experiments • All runs

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Sample: NRCM from Rattus norvegicus

SAMN03571557 • SRS924991 • All experiments • All runs

Organism: Rattus norvegicus

Library:

Name: neonatal rat cardiomyocytes

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: GENOMIC

Selection: unspecified

Layout: PAIRED

Spot descriptor:

1 forward51 reverse

Runs: 3 runs, 42.1M spots, 4.2G bases, 1.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR2002794	15,396,484	1.5G	640.4Mb	2016-04-29
SRR2002795	12,368,524	1.2G	515.3Mb	2016-04-29
SRR2002796	14,335,670	1.4G	595.2Mb	2016-04-29

ID:: 1468129

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